pGLO Lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 1 huge colony faded white faded white - pGLO LB/amp 0 white white + pGLO LB/amp 10 white white + pGLO LB/amp/ara 15 bright white faded green Neon green

2.	What two new traits do your transformed bacteria have? one trait is that it is resistant to ampicillin the other is that it glows green under UV light.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic. in the trillions because one e-coli is one micrometer cubed. There are 100 microleters on a plate. that totals up to an equal to 100 trillion e-coli
4.	What is the role of arabinose in the plates? The arabinose is a sugar which makes the e-coli glow a neon green color
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science. There have genetically engineered fish that are able to glow green under flurescent light. scientists have made away to make glowing genes that made a pigs nose turn neon orange scientists have made away to have glowing chickens
6.	Give an example of another application of genetic engineering. scientists have made a bionic chip which hooks up to the nerve system in the mody os small animals and are able to control them.

Candy Electrophoresis Lab

1)  Our experimental dyes and the reference dyes did not match up the same. They were very close but no the same.a) The blue reference dye was larger than the experimental dye, while the the orange experimental dye was larger then the orange reference dye.b)The blue reference dye is a darker blue then the experimental blue, aswell as the orange experimental dye was a little darker than the reference dye.c)For the experimental dyes, the orange and yellow dyes are in the same row.d)no.2) THe fast green FCF because it looks articficial towards the others. 3) to make the owners think that its real vegies in the dog food instead of artificial ingriediants.5)the two factors are the length and the density.6) THe element that helps move the dyes through the gel is the current of electricity.7)Electricity becasue it sneds currents and makes it move and expand.8) you would go from 5000 to 600.

New Years Goal

1) My first goal is to really learn the material this semester. I don't care about the grades as long as I really understand it. My action plan for this goal is to really pay attention and focus in class. Also to learn from my mistakes and ask questions when confused.

2)My second goal is to be less annoying in class. My action plan for this is to only use my water bottle for drinking purposes. Also to be less energetic. I will also only talk when I am supposed to/ told to. I will also help in anyway I can.