pGLO Observations , Data Recording & Analysis
1.
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Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
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2.
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What two new traits do your transformed bacteria have?
one trait is that it is resistant to ampicillin the other is that it glows green under UV light.
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3.
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Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
in the trillions because one e-coli is one micrometer cubed. There are 100 microleters on a plate. that totals up to an equal to 100 trillion e-coli
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4.
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What is the role of arabinose in the plates?
The arabinose is a sugar which makes the e-coli glow a neon green color
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5.
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List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
There have genetically engineered fish that are able to glow green under flurescent light.
scientists have made away to make glowing genes that made a pigs nose turn neon orange
scientists have made away to have glowing chickens
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6.
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Give an example of another application of genetic engineering.
scientists have made a bionic chip which hooks up to the nerve system in the mody os small animals and are able to control them.
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